定制基因
公司擁有專業的基因合成服務專家團隊和技術設備,整合了先進的基因序列設計和優化技術,可根據客戶需求,為您合成任何長度的基因及各種困難基因。

服務訂購

基因長度

交貨周期

價格(元/bp)

備  注

<400 bp

5~8 工作日

350


400~1500 bp

5~8 工作日

1


1501~3000 bp

10~15 工作日

1


3001~4000 bp

15~20 工作日

1.3


4001~5000 bp

20~25 工作日

1.4


5001~6000 bp

20~25 工作日

1.4


≥6000 bp

商  議

請詢價


困難基因

商  議

請詢價



◆ 如果客戶不指定載體,公司默認把客戶目的基因序列克隆到PUC57上,不收取亞克隆費用。

服務優勢
  • 周期短
  • 可靠先進的基因合成技術
  • 天衣無縫的無痕克隆方案
  • 精準的表達系統優化
  • 完善的項目管理
服務說明
    備注:
    1、基因長度:基因序列長度小于300bp的均按300bp的收費。
    2、預期克隆載體:請在訂單表或服務合同中注明。
    3、基因特性
    ①是否有嚴重的重復序列:比如一個片段重復若干次;或者100bp內,重復序列長度超過40bp;或者100bp內反向互補序列長度超過30bp。
    ②基因的GC含量情況:GC含量是否<30%或>70%(常規基因:指基因片段整體或部分的GC含量在30%-70%之間)。
服務流程
服務訂購
我公司為客戶提供全部技術資料和產品,包括:
-COA文件 -質粒結構圖
-約5μg凍干質粒DNA -測序圖譜
-含有重組質粒的甘油菌或穿刺菌一管

特別聲明:本公司承諾對客戶的基因序列不做任何形式的擴散、開放或利用,對相關信息實行完全保密,公司交付產品一個月后,徹底銷毀所有相關的引物、質粒和菌種

Peer-reviewed Citations:
Combined SNPs and miRNAs technologies for beef traceability

Q Wu, G Zhou, S Yang, X Xu, C Li - Journal of Food Safety ... Premix Ex Taq Kits (Takara Biotechnology Co. Ltd., Dalian, China). Forward primers used for RT-PCR were synthesized by General Biosystems (General Biosystems Co. Ktd., Anhui, China). The forward primer sequences of the...

Assessment of the fusion tags on increasing soluble production of the active TEV protease variant and other target proteins in E. coli

Xuelian Yu, Jiaqi Sun, Weiyu Wang, Li Jiang, Beijiu Cheng, Jun Fan-Applied Biochemistry and Biotechnology ...Primary and secondary antibodies as well as reagents for Western blotting were provided by Sangon Biotech (Shanghai, China). Primer synthesis and DNA sequencing were conducted by General Biosystems (Chuzhou, China)...

Engineering thermostable (R)-selective amine transaminase from Aspergillus terreus through in silico design employing B-factor and folding free energy calculations

J Huang, DF Xie, Y Feng - Biochemical and Biophysical Research Communications ...The AT-ATA cDNA sequence containing the NcoI and XhoI restriction sites was synthesized at General Biosystems (AnHui) Co., Ltd (Chuzhou, China), and the pET28a(+) expression vector(pET28a(+)-AT-ATA) was constructed as a template for site-directed mutagenesis...

A Vector Based on the Chicken Hypersensitive Site 4 Insulator Element Replicates Episomally in Mammalian Cells

Zhang,Xi;Zhang,Xi;Wang,Xiao-Yin;Wang,Xiao-Yin;Jia,Yan-Long;Jia,Yan-Long;Guo,Xiao;Guo, Xiao;Wang,Yan-Fang;Wang,Yan-Fang;Wang,Tian-Yun;Wang,Tian-Yun-Current Gene Therapy ...trans- gene expression. MATERIALS AND METHODS Vector Construction The cHS4 DNA sequence used in this study was artificially synthesized by General Biosystems, Inc. (Chuzhou, China) [23]. The synthesized sequences...

TTF1, in the Form of Nanoparticles, Inhibits Angiogenesis, Cell Migration and Cell Invasion In Vitro and In Vivo in Human Hepatoma through STAT3 Regulation

Bin Xiao, Dongjing Lin, Xuan Zhang, Meilan Zhang and Xuewu Zhang-Molecules …pcDNA3.1 plasmid of STAT3 was synthesized by General Biosystems (An Hui, General Biosystems, Inc., Chuzhou, China)...

Matrix attachment region combinations increase transgene expression in transfected Chinese hamster ovary cells

Chun-Peng Zhao, Xiao Guo, Si-Jia Chen, Chang-Zheng Li, Yun Yang, Jun-He Zhang, Shao-Nan Chen, Yan-Long Jia & Tian-Yun Wang-Sci Rep. …a 2073-bp fragment (position 94–2166) was synthesized by General Biosystems (Chuzhou, China)…

Genome-wide analysis of Dof family genes and their expression during bud dormancy in peach (Prunus persica)

M Chen, X Liu, L Huan, M Sun, L Liu, X Chen, D Gao, L Li-Scientia Horticulturae …All of the primerswere designed using the Beacon Designer 7 software and synthesized by GeneralBiosystems (Anhui, China)…

Improving thermostability of (R)-selective amine transaminase from Aspergillus terreus through introduction of disulfide bonds

DF Xie, H Fang, JQ Mei, JY Gong, HP Wang, XY Shen, J Huang, LH Mei-Biotechnology and Applied Science … Materials The AT-ATA cDNA from A. terreus sequence, including the NcoI and XhoI restriction sites, was synthesised at General Biosystems (AnHui) Co., Ltd (Chuzhou, China), and plasmid pET28a(+) was used for gene cloning and DNA sequencing...

Tobacco etch virus protease mediating cleavage of the cellulose-binding module tagged colored proteins immobilized on the regenerated amorphous cellulose

Xuelian YuJiaqi SunWeiyu WangLi JiangRuyue WangWenjun XiaoBeijiu ChengJun Fan-Bioprocess and Biosystems Engineering ... Materials and methods. Materials. Reagents used in plasmid construction and protein expression were bought from Takara (Dalian, China). Gene and primer syntheses, DNA sequencing are conducted by General Biosystems (Chuzhou, China)...
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